Journal: Experimental Biology and Medicine

Article Title: Liraglutide prevents fast weight gain and β-cell dysfunction in male catch-up growth rats

doi: 10.1177/1535370214567614

Figure Lengend Snippet: Effects of HFD catch-up growth and liraglutide treatment on β-cell function and apoptosis. Pdx-1 protein (a1) and mRNA (a2) levels of islets at the end of week 8. Pdx-1 protein levels of islets were assessed by Western blot and the results were expressed as the ratios of Pdx-1 and GAPDH. The Pdx-1 mRNA levels were detected by qPCR and expressed as the ratios of Pdx-1 and β-actin. Protein levels of Bcl-2 (b1) and Caspase-3 p 12 subunit (c1) were detected by Western blot and the results were expressed as the ratios of Caspase-3 p12 subunit or Bcl-2 with GAPDH. The mRNA levels of Bcl-2 (b2) and Procaspase-3 (c2) were expressed as the ratios of Proaspase-3 or Bcl-2 with β-actin. All the results are expressed as mean ± SEM. *p < 0.01 versus the NC group; #p < 0.01 versus the CUG group; &p < 0.01 versus the CUGL group

Article Snippet: Primer sequences Western blot analysis Protein extracts from islets (20 μg from each rat) were subjected to Western blot analysis using mouse antipancreatic duodenal homeobox-1 (Pdx-1) monoclonal antibody (1:800; Cell Signaling Technology, Danvers, MA, USA), mouse anti-Caspase-3 p12 subunit monoclonal antibody (1:500, Boster Bio-Engineering, China), mouse anti-B-cell lymphoma (Bcl-2) polyclonal antibody (1:800, Bioworld, USA), and mouse anti-GAPDH antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Cell Function Assay, Western Blot